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human asb7  (Addgene inc)


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    Structured Review

    Addgene inc human asb7
    Fig. 4 | ITC analysis of the key elements required for <t>ASB7</t> recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1
    Human Asb7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+asb7/pRL-TK+4x+mut+E+(Plasmid+%2311318)/pm39039081-160-5-25
    Average 92 stars, based on 1 article reviews
    human asb7 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase."

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.

    Journal: Nature communications

    doi: 10.1038/s41467-024-50556-x

    Fig. 4 | ITC analysis of the key elements required for ASB7 recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1
    Figure Legend Snippet: Fig. 4 | ITC analysis of the key elements required for ASB7 recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1

    Techniques Used: Binding Assay

    Related Articles

    Expressing:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Purification:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Amplification:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase
    Article Snippet: .. The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28-MKH8SUMO vector (Addgene plasmid #79526). ..

    Polymerase Chain Reaction:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase
    Article Snippet: .. The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28-MKH8SUMO vector (Addgene plasmid #79526). ..

    cDNA Library Assay:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase
    Article Snippet: .. The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28-MKH8SUMO vector (Addgene plasmid #79526). ..

    Clone Assay:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase
    Article Snippet: .. The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28-MKH8SUMO vector (Addgene plasmid #79526). ..

    Plasmid Preparation:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase
    Article Snippet: .. The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28-MKH8SUMO vector (Addgene plasmid #79526). ..



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    Fig. 4 | ITC analysis of the key elements required for <t>ASB7</t> recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1
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    Fig. 4 | ITC analysis of the key elements required for <t>ASB7</t> recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1
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    Image Search Results


    Fig. 4 | ITC analysis of the key elements required for ASB7 recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1

    Journal: Nature communications

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.

    doi: 10.1038/s41467-024-50556-x

    Figure Lengend Snippet: Fig. 4 | ITC analysis of the key elements required for ASB7 recognition. a ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 1 mutants. NB, no apparent binding under our experimental conditions. b ITC fitting curves of LZTS1 peptide titrated to wild-type ASB7 and site 2/3 mutants. c ITC fitting curves of LZTS1

    Article Snippet: Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526).

    Techniques: Binding Assay